Mammalian cell culture has long been an invaluable tool in cell biology, drug discovery, and regenerative medicine. When cell culture techniques were first developed, 3-Dimensional (3D) systems were utilized. That rapidly changed due to cost and efficiency concerns resulting in cell culture being performed today with adherent cells grown on flat and rigid two-dimensional (2D) substrates, including polystyrene or glass. Advances in our understanding of cell physiology and failures in clinical trials have provided the impetus to move away from 2D systems and back to a more in-vivo-like 3D environment. The advance of these new technologies and screening methodologies have allowed scientists to assess more realistic functional capabilities of cells. The following course will focus on the cellular microenvironment and its importance when developing and screening cell-based assays using primary, stem cell, and immortalized cultures in 3D systems.
Those interested in the following tracks:
Mark E. Rothenberg Ph.D.
Mark Rothenberg graduated from Emory University with his PhD in Cell and Developmental Biology with a focus in Comparative and Molecular Endocrinology. Over the past 25 years Mark has held positions in both Academia and industry where he has developed an expertise in the areas of assay development and cell culture. Mark has worked for Corning Incorporated for 10 years and prior to his current position as Manager Scientific Training and Education he was the team lead for the Applications team, where his work led to many presentations at numerous conferences across the North America, Europe, and Asia.
Brad is a Principal Scientist at BioTek Instruments, INC., where he has worked since 2009. Prior to joining BioTek, he had extensive experience with multiple reagent providers working in various capacities. His current roles include optimizing new assay processes on BioTek’s line of automation, liquid handling, microplate detection, and imaging instrumentation. He has worked for more than 20 years with numerous automation and detection platforms, and a variety of cell models, to optimize a variety of assays using 2D and 3D cell culture across multiple research fields. His current work has led to publications in Assay and Drug Development Technologies, The Journal of Laboratory Automation, The Journal of Biomolecular Screening, and Combinatorial Chemistry and High Throughput Screening, among others. He has also presented his work at numerous conferences across the United States, Europe, and Asia.