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SLAS2018 Short Courses

Set-up and Validation of 3D Primary, Stem Cell and Immortalized Cultures for Downstream Microplate Reader and Imaging Applications (NEW!)

Mammalian cell culture has long been an invaluable tool in cell biology, drug discovery, and regenerative medicine. When cell culture techniques were first developed, 3-Dimensional (3D) systems were utilized. That rapidly changed due to cost and efficiency concerns resulting in cell culture being performed today with adherent cells grown on flat and rigid two-dimensional (2D) substrates, including polystyrene or glass. Advances in our understanding of cell physiology and failures in clinical trials have provided the impetus to move away from 2D systems and back to a more in-vivo-like 3D environment. The advance of these new technologies and screening methodologies have allowed scientists to assess more realistic functional capabilities of cells. The following course will focus on the cellular microenvironment and its importance when developing and screening cell-based assays using primary, stem cell, and immortalized cultures in 3D systems.

Who Should Attend:

  • Industry and academic scientists
  • Those interested in or currently involved in 3D culture systems
  • Those with mid to advanced-level experience in:
    • Cell-based assays
    • Experienced in using liquid handling, microplate reading and imaging technologies

Those interested in the following tracks:

 

How You Will Benefit From This Course:

  • Helpful hints for setting up, validating, and assessing a variety of cell types in a 3D environment
  • Utilizing automation in setting up, validating, and screening 3D cultures
  • A focused discussion on assay types and microplate reading and imaging techniques that are currently in use for screening in 3D

Course Topics:

  • Introduction
    • Understanding the cell-based assay microenvironment
      • Importance of the chosen platform
      • Medium considerations
      • Surface and ECM’s
    • 2-Dimensional vs 3-Dimensional cell culture: Compare and Contrast
    • 3-Dimensional Systems: Evolution of Historical to Current Methods
      • Primary cultures
      • Stem Cell Cultures
      • Immortalized Cultures
    • Summary
  • Setting up and validating 3D systems
    • Optimizing the 3D microenvironment
      • Media
      • ECM’s
      • Cells
    • Automating the process
      • Liquid handling
      • Microplate/system handling>
    • An introduction to assaying in 3D
      • What techniques are available to screen 3D systems
        • Microplate based readers
          • Systems used in 2D and 3D
          • Assaying Kits
            • Traditional kits for assaying 2D
            • Advanced methods; 2D kits being used for 3D applications
            • Kits developed for 3D
        • Imaging
          • Systems used in 2D and 3D
          • Assaying Kits
            • Traditional techniques for assaying 2D
            • Advanced methods; 2D kits being used for 3D applications
            • Kits developed for 3D
      • Considerations when setting up 3D assays
        • Reading and imaging using current 3D technologies
        • Setting up the assay using LH and automation in a 3D environment
    • Summary
  • Current issues with 3D assay systems and ways/methods to overcome them
    • Summary of course
    • Group discussion focused current issues involved in culturing and assaying in 3D

Instructors:

Mark E. Rothenberg Ph.D.

Mark E. Rothenberg Ph.D.
Manager Scientific Training and Education
Corning Incorporated I Life Sciences

Mark Rothenberg graduated from Emory University with his PhD in Cell and Developmental Biology with a focus in Comparative and Molecular Endocrinology. Over the past 25 years Mark has held positions in both Academia and industry where he has developed an expertise in the areas of assay development and cell culture. Mark has worked for Corning Incorporated for 10 years and prior to his current position as Manager Scientific Training and Education he was the team lead for the Applications team, where his work led to many presentations at numerous conferences across the North America, Europe, and Asia.

Brad Larson

Brad Larson
Principal Scientist
BioTek Instruments, Inc.

Brad is a Principal Scientist at BioTek Instruments, INC., where he has worked since 2009. Prior to joining BioTek, he had extensive experience with multiple reagent providers working in various capacities. His current roles include optimizing new assay processes on BioTek’s line of automation, liquid handling, microplate detection, and imaging instrumentation. He has worked for more than 20 years with numerous automation and detection platforms, and a variety of cell models, to optimize a variety of assays using 2D and 3D cell culture across multiple research fields. His current work has led to publications in Assay and Drug Development Technologies, The Journal of Laboratory Automation, The Journal of Biomolecular Screening, and Combinatorial Chemistry and High Throughput Screening, among others. He has also presented his work at numerous conferences across the United States, Europe, and Asia.


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